Iclip genetics11/19/2022 ![]() ![]() Only complete applications will be considered. Abstract of your current research (max.explain why you would like to attend, including what you can contribute and how you think you will benefit from the practical course.include your relevant skills, experience and qualifications which show that you would be suitable for the practical course.We will prioritise applications from junior researchers at the PhD and early postdoc stage.Īpplicants are required to provide the following information:.iCLIP or related technologies (Hits-CLIP, PAR-CLIP, ribosome profiling) as part of their project in the near future. participants should be likely to use e.g. The course content should be relevant to their research, i.e.Participants should have experience in experimental RNA biology.The course is limited to 18 participants, which will be selected according to the following criteria: Payment details will be sent to successful applicants. #ICLIP GENETICS FULL#full catering (breakfast, lunch, dinner, refreshments).The meeting environmentally more sustainable. Organisers are encouraged to implement measures to make Participants and speaker gender diversity (at least 40% of speakers must be female). The hands-on sessions in the laboratory will be complemented by presentations by established scientists that will share their experience on using iCLIP to answer different biological questions.ĮMBO Courses and Workshops are selected for their excellent scientific quality and timeliness, provision of good networking activities for all We will further discuss the bioinformatics strategies for analysis of iCLIP data. We will guide the participants through the complete protocol, placing special emphasis on critical optimisation steps and controls. The major objective of this EMBO Practical Course is to disseminate the knowledge and proficiency in this technically challenging ribonomics method. Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) has established itself as a leading method to map the RNA interaction sites of a single RBP-of-interest with high resolution and in a quantitative manner. Understanding these interactions in a genome-wide manner is crucial for gaining a complete picture of an RBP’s specificity and function. In turn, RBPs can interact with hundreds to thousands of cellular RNAs by recognizing certain sequence motifs or structural elements. Network analysis showed that identified targets containing HuR binding sites in the 3′ UTR are highly interconnected.Throughout its life cycle, each RNA will interact dynamically with numerous RNA-binding proteins (RBPs) that determine how the RNA is processed, where it is localized, whether it is translated and finally how it is degraded. HuR sites in 3′-UTRs overlap extensively with predicted microRNA target sites, suggesting interplay between the functions of HuR and microRNAs. Despite the fact that HuR sites are observed in intronic regions, our data do not support a role for HuR in regulating splicing. We reveal that HuR targets predominantly uracil-rich single-stranded stretches of varying size, with a strong conservation of structure and sequence composition. We take an important step in this direction by conducting cross-linking and immunoprecipitation and RNA sequencing experiments followed by an extensive computational analysis to determine the characteristics of the HuR binding site and impact on the transcriptome. Despite a great deal of prior investigation into HuR, there is still much to learn about its function. The ubiquitously expressed RNA-binding protein Hu antigen R (HuR) or ELAVL1 is implicated in a variety of biological processes as well as being linked with a number of diseases, including cancer. Glycobiology and Extracellular Matrices. ![]()
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